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Email: info@ccnbio.comRead MoreGlutathione Agarose 4FF/4N is an affinity medium prepared by conjugating glutathione ligand to an agarose matrix, which is mainly used for the purification of glutathione S-transferase and GST fusion proteins, and can obtain high-purity target proteins in one step. Product Type: Prepacked columns, Resins Pack Size: 1mL 5mL 10mL 25mL 100mL 500mL 1L 5L 10L -
Email: info@ccnbio.comRead MoreChelating Agarose FF and Chelating Agarose HF are metal chelate affinity chromatography media prepared by coupling iminodiacetic acid (IDA) to agarose. Chelating Agarose HF is characterized by greater rigidity, higher pressure resistance, and faster flow rates compared to Chelating Sepharose FF. Iminodiacetic acid (IDA) provides three coordination sites for chelating metal ions, which in turn offer three binding sites for the specific adsorption of target proteins. Under identical ligand density and metal ion conditions, both Chelating Agarose FF and Chelating Agarose HF exhibit stronger affinity interactions compared to IMAC (Immobilized Metal Affinity Chromatography) Agarose FF. Once charged with metal ions such as Cu(2+), Zn(2+), Ni(2+), Co(2+), etc., these media can be used for the purification and separation of recombinant and natural proteins. Product Type: Prepacked columns, Resins Pack Size: 1mL 5mL 10mL 25mL 100mL 500mL 1L 5L 10L -
Email: info@ccnbio.comRead MoreThe IMAC Agarose FF and IMAC Agarose HP are metal chelating affinity chromatography media prepared by coupling nitrilotriacetic acid (NTA) to 6% highly cross-linked agarose. These media, which are not pre-chelated with metal ions, can provide four coordination sites for metal ion chelation, resulting in a more stable binding of the chelated metal ions and allowing them to withstand certain concentrations of reducing agents. After users chelate metal ions such as Cu(2+), Zn(2+), Ni(2+), Co(2+), etc., these media can be used for the separation and purification of recombinant proteins and natural proteins. Product Type: Prepacked columns, Resins Pack Size: 1mL 5mL 10mL 25mL 100mL 500mL 1L 5L 10L
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Email: info@ccnbio.comCCN Ni Agarose FF is an affinity chromatography medium designed for the purification of histidine-tagged proteins, pre-packed with nickel ions firmly bound to a chelating ligand. When chelating agents are present in the sample, the medium minimizes nickel ion leaching while maximizing protein recovery. It is suitable for purifying proteins expressed in eukaryotic cells like insect-baculovirus, 293 cells, and CHO cells, as well as in bacteria such as E. coli. The medium allows direct loading of samples without pre-treatment, preventing nickel leaching. Ni Agarose FF / Ni Agarose HP affinity chromatography media are designed by chelating divalent nickel ions (Ni(2+)) onto an agarose gel matrix with aminotriacetic acid as the ligand. These media offer several advantages, including high adsorption capacity, good selectivity, ease of regeneration, and low cost. They are widely used for the separation and purification of proteins and peptides, particularly for purifying histidine-tag. Product Type: Prepacked columns, Resins Pack Size: 1mL 5mL 10mL 25mL 100mL 500mL 1L 5L 10LRead More
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Email: info@ccnbio.comRead MoreIgM/IgY Capture Agarose HP is an affinity medium prepared by coupling the thiol compound 2-mercaptopyridine to a highly cross-linked 6% agarose matrix, with an average particle size of 34μm. The principle of thiol-affinity chromatography is based on the interaction between electron donors and electron acceptors to purify biomolecules. This interaction is enhanced in high-salt environments and weakened in low-salt conditions. IgM/IgY Capture Agarose HP is primarily used for the rapid and efficient purification of IgM/IgY antibodies. Product Type: Prepacked columns, Resins Pack Size: 1mL 5mL 10mL 25mL 100mL 500mL 1L 5L 10L
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Email: info@ccnbio.comRead MoreProtein G agarose 4FF is an affinity chromatography medium prepared by coupling recombinant protein G to a highly cross-linked agarose matrix. It offers a broader binding spectrum compared to protein A, demonstrating enhanced binding capacity for IgG. Protein G not only binds to the Fc fragment of antibodies but also exhibits a weak interaction with the Fab fragment. Consequently, Protein G Agarose 4FF is commonly used for the separation and purification of antibodies or antibody fragments from cell culture supernatants or cell extracts, as well as for purifying polyclonal antibodies from sera of various animal species. Product Type: Prepacked columns, Resins Pack Size: 1mL 5mL 10mL 25mL 100mL 500mL 1L 5L 10L -
Email: info@ccnbio.comProtein A Resins Protein A Agarose HL is prepared by coupling a robust alkaline-resistant Protein A ligand to a high-rigidity agarose matrix. This resin exhibits excellent ligand stability, minimal ligand leaching during purification, and facilitates easy removal of residual ligands in downstream processes. It can be efficiently cleaned in place (CIP) with 0.1⁓0.5M NaOH, eliminating the need for expensive and corrosive CIP reagents, thereby providing cost savings. It also offers good flow rates, pressure tolerance, and scalability, making it an ideal choice for monoclonal antibody purification. Feature:s High loading capacity, strong alkali resistance, fast flow rate Product Type: Prepacked columns, Resins Pack Size: 1mL 5mL 10mL 25mL 100mL 500mL 1L 5L 10LRead More -
Email: info@ccnbio.comRead MoreThe CCN 293 series serum-free cell culture media are general-purpose serum-free media suitable for applications ranging from laboratory research and development to industrial-scale production. This series of media can be adapted to various 293 cell lines and can be used in conjunction with multiple transfection reagents to achieve efficient protein expression and effective viral vector packaging Features: Serum-Free, Animal Component-Free, Chemically Defined Product Type: Liquid, Powder Pack Size: 50mL 100mL 500mL 1L 5L 10L 50L 100L Customized -
Email: info@ccnbio.comCCN CHO series cell culture medium and feed is a general-purpose serum-free CD medium, which is suitable for a variety of engineered cell lines based on CHO-K1 background (ATCC, CHOZN®, Lonza, Horizon, etc.), which can meet the efficient expression of target products under fed-batch and high-density fed-batch culture processes. Features: Serum-Free, Animal Component-Free, Chemically Defined Product Type: Liquid, Powder, Basal, Feed Pack Size: 10mL, 35mL, 100mL, 350mL, 500mL, 1L, 5L, 10L, 50L, 100L, CustomizedRead More