Description
CCN Bio Micrococcal Nuclease (MNase) is a recombinant endo-exonuclease derived from Staphylococcus aureus that efficiently degrades single-stranded and double-stranded DNA, RNA, chromatin, and nucleoprotein complexes. The enzyme preferentially cleaves nucleic acids at AT-rich regions, generating oligonucleotides with 3′-phosphate and 5′-hydroxyl termini.
Micrococcal Nuclease is widely used in chromatin biology, epigenetics, molecular biology, and protein purification workflows. It is particularly useful for chromatin digestion, nucleosome mapping, removal of contaminating nucleic acids from protein preparations, and preparation of samples for downstream sequencing and structural biology applications.
CCN Bio Micrococcal Nuclease is calcium-dependent and exhibits robust activity under physiological conditions. The enzyme can be readily inactivated by chelating agents such as EGTA or EDTA, allowing precise control of digestion reactions.
This product is supplied as a highly purified recombinant enzyme suitable for research applications.
Features
- Efficient digestion of DNA and RNA
- Active on single-stranded and double-stranded nucleic acids
- Effective chromatin and nucleosome digestion
- Calcium-dependent activity allows controlled reactions
- Easily inactivated by EDTA or EGTA
- High purity and low protease contamination
- Suitable for chromatin, epigenetics, and structural biology applications
Applications
- Chromatin digestion and nucleosome mapping
- MNase-seq sample preparation
- Chromatin accessibility studies
- Epigenetic and transcriptional regulation research
- Removal of nucleic acid contamination from protein samples
- Preparation of protein complexes for structural biology
- Reduction of lysate viscosity during protein purification
Product Performance
| Parameter | Specification |
| Source | Recombinant Staphylococcus aureus Micrococcal Nuclease |
| Enzyme Type | Endo-Exonuclease |
| Substrates | ssDNA, dsDNA, RNA, Chromatin |
| Cofactor Requirement | Ca²⁺ |
| Optimal pH | 7.5–9.0 |
| Optimal Temperature | 37°C |
| Inactivation | EDTA or EGTA |
| Protease Contamination | Not Detectable |
| Storage Temperature | -20°C |
Unit Definition
One unit is defined as the amount of enzyme required to produce an increase in absorbance equivalent to complete digestion of 1 μg of calf thymus DNA in 30 minutes at 37°C under specified assay conditions.
Ordering Information
| Product | Catalog Number | Size |
| CCN Bio Micrococcal Nuclease | CCNMN0010 | 10,000 Units |
| CCN Bio Micrococcal Nuclease | CCNMN0050 | 50,000 Units |
| CCN Bio Micrococcal Nuclease | CCNMN0100 | 100,000 Units |
| CCN Bio Micrococcal Nuclease | CCNMN0500 | 500,000 Units |
| CCN Bio Micrococcal Nuclease | CCNMN1000 | 1,000,000 Units |
Customized Packaging Available
Additional package sizes, bulk quantities, OEM packaging, custom concentrations, and GMP-compatible manufacturing formats are available upon request.