Description
A high-performance nucleic acid removal enzyme — a powerful alternative to Benzonase®
What Is CCN EndoCleava™?
CCN EndoCleava™ Nuclease is a fast and efficient recombinant endonuclease used to remove unwanted DNA and RNA from biological samples. Produced in E. coli, it is designed for both everyday research and large-scale bioprocessing.
A Next-Generation Alternative to Benzonase®
EndoCleava™ delivers the same core functionality people expect from Benzonase®—rapid digestion of nucleic acids and reduction of sample viscosity—while offering high purity, strong activity, and excellent compatibility with common bioprocess buffers.
It is fully capable of replacing Benzonase® in most workflows, including viral vector production, protein purification, and cell culture processing.
(Benzonase® is a registered trademark of Merck KGaA and is referenced here only for product comparison.)
How It Works
EndoCleava™ breaks down all forms of DNA and RNA—including linear, circular, single-stranded, double-stranded, and supercoiled structures—into very small fragments.
This improves sample clarity, reduces filter clogging, and creates smoother downstream purification.
Why Choose EndoCleava™?
- High Activity
Quickly digests nucleic acids and significantly reduces sample viscosity—even in dense lysates.
- Protein-Safe
Contains no detectable protease activity, so your proteins stay intact throughout purification.
- Broad Compatibility
Operates effectively across a wide range of pH, temperatures, salts, detergents, and reducing agents.
- Scalable for Bioprocessing
Suitable for viral vector manufacturing, vaccine development, and recombinant protein production.
- Easy Benzonase® Replacement
Use in similar buffer conditions, reaction setups, and unit definitions—making transition seamless.
Applications
CCN EndoCleava™ is ideal for:
- AAV, lentiviral, and adenoviral vector manufacturing
- Vaccine purification workflows
- Monoclonal antibody and protein drug purification
- Host-cell DNA removal during downstream processing
- Reduction of cell paste or lysate viscosity
- Improving filterability and centrifugation efficiency
Sample cleanup prior to mass spectrometry and gel electrophoresis
Key Advantages at a Glance
- Rapid DNA/RNA digestion
- No protease activity
- High purity and low endotoxin
- Works in tough samples and crowded buffers
Drop-in replacement for Benzonase® in many protocols
Summary
CCN EndoCleava™ Nuclease provides a high-activity, protein-safe, and broadly compatible solution for nucleic acid removal. Designed as a modern alternative to Benzonase®, it supports cleaner samples, smoother purification, and more efficient bioprocessing across research and industrial workflows.
Order information
Available in standard and customizable formats
CCN EndoCleava™ Nuclease is offered in multiple activity sizes to support everything from routine research to large-scale bioprocessing. Whether you are running small pilot experiments or managing full production runs, we provide flexible packaging tailored to your workflow.
Standard Sizes
Choose from our most popular formats:
- 10 kU – Ideal for small-scale experiments or assay development
- 50 kU – Suitable for routine lab use and mid-scale workflows
- 100 kU – Great for pilot runs or process optimization studies
- 500 kU – Cost-effective for high-volume or production environments
Custom Packaging Available
We also offer fully customizable packaging, including:
- Custom activity levels (1 kU to multi-million units)
- Bulk packaging for GMP or industrial use
- Concentrated formulations
- Private-label or OEM supply
- Special buffer compositions upon request
If your workflow requires unique specifications, our team can prepare a tailored packaging solution.
Bulk Orders & OEM Supply
For large-scale manufacturing needs, CCN Bio provides:
- Discounted pricing on bulk quantities
- Consistent lot-to-lot performance
- Long-term supply agreements
- Custom QC specifications to meet process requirements