Ni Resins

CCN Ni Agarose FF is an affinity chromatography medium designed for the purification of histidine-tagged proteins, pre-packed with nickel ions firmly bound to a chelating ligand. When chelating agents are present in the sample, the medium minimizes nickel ion leaching while maximizing protein recovery. It is suitable for purifying proteins expressed in eukaryotic cells like insect-baculovirus, 293 cells, and CHO cells, as well as in bacteria such as E. coli. The medium allows direct loading of samples without pre-treatment, preventing nickel leaching. Ni Agarose FF / Ni Agarose HP affinity chromatography media are designed by chelating divalent nickel ions (Ni(2+)) onto an agarose gel matrix with aminotriacetic acid as the ligand. These media offer several advantages, including high adsorption capacity, good selectivity, ease of regeneration, and low cost. They are widely used for the separation and purification of proteins and peptides, particularly for purifying histidine-tag. Product Type: Prepacked columns, Resins Pack Size: 1mL 5mL 10mL 25mL 100mL 500mL 1L 5L 10L