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CCNBio
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"We love what we do and we love helping others succeed at what they love to do."
Protein reagents – Protein A and Protein G
Only commercial production that is 100%-free of all animal and plant extracts
Produced using glucose/salts minimal media
High quality and documented lot-to-lot reproducibility
Very competitive bulk-order pricing
Variant gene synthesis/protein expression and purification
Optimization of expression and protein yield
Custom expression in bacterial, yeast, and mammalian systems
Western blot analysis
Maldi-MS
Small scale protein production
Optimize expression conditions
Evaluate multiple variants for expression and activity
Expression conditions for seamless scale-up to large scale production
Large scale protein production:
10L – 130L batch sizes
Up to “kilogram”-scale capacity
Bacterial expression with proprietary CleanGenome® strains
High expression with optimized conditions for each protein
Removed E coli genes that gluconate proteins (BL-21 gluconates)
Extremely tight and proprietary regulation of transcription, aids in expression of difficult (i.e. toxic) proteins
CleanGenome® strains contain *no* IS elements, transposons or prophage sequences
Extremely reduced protease & nuclease activity
Yeast expression
Pichia – variant human proteins used as controls in therapeutic clinical trials
Saccharomyces – alternative post-translational processing
Recombinant monoclonal antibody expression in mammalian cell lines including CHO and HEK293
Single Chain Antibodies (scFV)
Extensive work on multiple DARPA scFv projects.
Production of thousands of scFV variants
Development and optimization of binding specificities
Custom protein modifications (i.e. biotinylation, cross-linking, fluorophores, etc.)
Assay development
ELISA & MSD-ECL binding assays
Develop and optimize assays de novo
Convert existing assays between ELISA & MSD-ECL platforms
Assay optimization for sensitivity, specificity and reproducibility
FACS (Fluorescence-activated cell sorting) assays
Custom-labeled antibodies
Biotinylation
ECL tags
Fluorescent labels
Cell-based assays
Gene cloning and expression
Confirmation of vector integration
Real-time PCR
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