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  • Protein reagents – Protein A and Protein G
    • Only commercial production that is 100%-free of all animal and plant extracts
    • Produced using glucose/salts minimal media
    • High quality and documented lot-to-lot reproducibility
    • Very competitive bulk-order pricing
  • Variant gene synthesis/protein expression and purification
    • Optimization of expression and protein yield
    • Custom expression in bacterial, yeast, and mammalian systems
    • Western blot analysis
    • Maldi-MS
  • Small scale protein production
    • Optimize expression conditions
    • Evaluate multiple variants for expression and activity
    • Expression conditions for seamless scale-up to large scale production
  • Large scale protein production:
    • 10L – 130L batch sizes
    • Up to “kilogram”-scale capacity
  • Bacterial expression with proprietary CleanGenome® strains
    • High expression with optimized conditions for each protein
    • Removed E coli genes that gluconate proteins (BL-21 gluconates)
    • Extremely tight and proprietary regulation of transcription, aids in expression of difficult (i.e. toxic) proteins
    • CleanGenome® strains contain *no* IS elements, transposons or prophage sequences
    • Extremely reduced protease & nuclease activity
  • Yeast expression
    • Pichia – variant human proteins used as controls in therapeutic clinical trials
    • Saccharomyces – alternative post-translational processing
  • Recombinant monoclonal antibody expression in mammalian cell lines including CHO and HEK293
  • Single Chain Antibodies (scFV)
    • Extensive work on multiple DARPA scFv projects.
    • Production of thousands of scFV variants
    • Development and optimization of binding specificities
  • Custom protein modifications (i.e. biotinylation, cross-linking, fluorophores, etc.)
    • Assay development
    • ELISA & MSD-ECL binding assays
    • Develop and optimize assays de novo
    • Convert existing assays between ELISA & MSD-ECL platforms
    • Assay optimization for sensitivity, specificity and reproducibility
    • FACS (Fluorescence-activated cell sorting) assays
    • Custom-labeled antibodies
      • Biotinylation
      • ECL tags
      • Fluorescent labels
    • Cell-based assays
  • Gene cloning and expression
    • Confirmation of vector integration
    • Real-time PCR
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