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Ni Resins

Email: info@ccnbio.com

CCN Ni Agarose FF is an affinity chromatography medium designed for the purification of histidine-tagged proteins, pre-packed with nickel ions firmly bound to a chelating ligand. When chelating agents are present in the sample, the medium minimizes nickel ion leaching while maximizing protein recovery. It is suitable for purifying proteins expressed in eukaryotic cells like insect-baculovirus, 293 cells, and CHO cells, as well as in bacteria such as E. coli. The medium allows direct loading of samples without pre-treatment, preventing nickel leaching.

Ni Agarose FF / Ni Agarose HP affinity chromatography media are designed by chelating divalent nickel ions (Ni(2+)) onto an agarose gel matrix with aminotriacetic acid as the ligand. These media offer several advantages, including high adsorption capacity, good selectivity, ease of regeneration, and low cost. They are widely used for the separation and purification of proteins and peptides, particularly for purifying histidine-tag.

Product Type: Prepacked columns, Resins

Pack Size: 1mL 5mL 10mL 25mL 100mL 500mL 1L 5L 10L

Category: Tag:

Description

Product CCN Ni Agarose FF Ni Agarose FF Ni Agarose HP
Features Resistant to chelating agents and reducing agents High adsorption capacity, good selectivity, and easy to regenerate
Application Suitable for purifying proteins expressed in eukaryotic cells like insect-baculovirus, 293 cells, and CHO cells, as well as in bacteria such as E. coli Purification of histidine-tagged proteins
Matrix 6% highly cross-linked agarose
Particle size 90 μm 90 μm 34 μm
Functional group Ni2+
Ligand concentration 50-90 μmol Ni2+/mL resin 20-25 μmol Ni2+/mL resin
Capacity 10mg His-tagged protein/mL resin 40mg His-tagged protein/mL resin
Flow velocity ＜250 cm/h ＜250 cm/h ＜150 cm/h
Max. pressure ＜0.3 MPa
pH stability 3-12(operational), 2-14(CIP，short term，when removing metal ions)
Chemical stability 0.01M HCl, 0.01M NaOH（one week）；10mM EDTA, 5mM DTT, 1M NaOH, 5mM TCEP, 20mM β-mercaptoethanol, 6M guanidine hydrochloride(24H); 500mM imidazole, 100mM EDTA(2H); 30% isopropanol(20 minutes) 40℃ for1 week：0.01M HCl, 0.1M NaOH, 8M urea, 6M guanidine hydrochloride;

12h：1M NaOH, 70% Acetic acid;

1h: 2% SDS; 30min: 30% Isopropyl alcohol
Storage conditions 20% ethanol; 4-30℃

 

Pack size Cat. No.
CCN Ni Agarose FF Ni Agarose FF Ni Agarose HP
1ml Prepacked columns CCNCNC001 CCNNIC001F CCNNIC001H
5ml Prepacked columns CCNCNC002 CCNNIC002F CCNNIC002H
5ml XS Prepacked columns CCNCNC003 CCNNIC003F CCNNIC003H
10ml CCNCNR010 CCNNIR010F CCNNIR010H
25ml CCNCNR025 CCNNIR025F CCNNIR025H
100ml CCNCNR100 CCNNIR100F CCNNIR100H
500ml CCNCNR500 CCNNIR500F CCNNIR500H
1L CCNCNR01L CCNNIR01LF CCNNIR01LH
5L CCNCNR05L CCNNIR05LF CCNNIR05LH
10L CCNCNR10L CCNNIR10LF CCNNIR10LH
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